Fig. 2. LELI enhances the number of satellite cells emanating from single fibers. Single myofibers prepared from EDL muscle of 21-day-old mice were non-irradiated (left panels) or irradiated with He-Ne laser (right panels) and kept in serum-free medium for the entire experiment. Data are expressed as the cumulative rank of data from single fibers of four independent experiments (n=200). Results are ranked according to the number of cells that accumulated around each single fiber on the indicated days. Individual ranks are expressed as percentage of total rank to normalize the data for sample size, plotted on the vertical axis. Superimposed on each curve as an H symbol is the mean value±1 s.e.m. for each treatment. Median value is indicated by dotted lines. In most cases, the mean and median values are close, therefore most samples are not distant from a Gaussian distribution. The population distribution of LELI cells is significantly different from control cells on all days (Kruskal-Wallis ANOVA median test; Rao R (4,99)=9.65; P<0.001).

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