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Fig. 4. Effect of LELI on anti- and pro-apoptotic proteins. Two fibers were plated
on each Petri dish: one was irradiated and the other served as a control. One
day post irradiation, fibers were fixed and stained for BAX (A, C) or Bcl-2
(B, D) and counter-stained with DAPI. Bar, 30 µM. (E) Western blot analysis
of Bcl-2, BAX, p53 and p21 in control and irradiated i28 myogenic cells on
various days post-irradiation. Equal quantities of proteins were loaded as
evidenced by staining for 
-tubulin. (F) Densitometric analysis of
protein expression levels normalized to that of -tubulin. Results are
averages of two independent repeats.
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