Fig. 5. Time-lapse experiments showed live the neural progenitor proliferation and differentiation into neurons as well as glia. (A) Transmitted light and fluorescence monitoring of isolated EB-derived cells. Starting from a single progenitor (arrow), characterized by its typical morphology and prominent EGFP expression, cell division (2.5 h, 6 h, 36.5 h) and differentiation into neuronal (unipolar, 11.5 h; bipolar, 12 h) cells (arrowhead) was observed in a representative experiment. Upon migration and further differentiation the EGFP fluorescence declined. (B) In line with the retained capacity of proliferation of glia (arrow), prior to division rounding-up of the glial cell was noticed (54.5 h). After division, migration, cellular interaction as well as flattening of the cells occurred. The cell labelled with arrowhead is likely of other origin. Bars, 30 µM (A); 50 µM (B).

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