Fig. 3. Analysis of GFP8 cells by standard and immunogold electron microscopy. (a-c) Postembedding immunogold electron microscopy. Sections were stained with anti-rabbit-IgG 10 nm gold and either anti-SFA (a) or anti-GFP (b and c, which are consecutive sections). Arrowheads in a and c show SMAFs, and arrowheads in b indicate the immunodecorated region between the two basal bodies at the origin of the SMAFs. (d-f) Three consecutive sections showing a basal apparatus with prominent SMAFs. Open arrows: microtubules originating on or near the SMAFs; arrows: two-stranded microtubular roots alongside the SMAFs; double arrowheads: new basal bodies. (g) Control cell showing new basal bodies (double arrowhead) and thin SMAFs (arrows), visible as electron-opaque bands along the microtubular roots. For better images of wild-type SMAFs see Goodenough and Weiss (Goodenough and Weiss, 1978). (h) Section through a GFP8 cell with prominent SMAF, secondary cytoskeletal microtubules (open arrow) and new basal body (double arrowhead). Bars, 1 µm.

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