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Fig. 2. Micrographs illustrating the different patterns of process formation induced by the expression of the MAP2b and MAP2c truncated forms in Sf9 cells. All truncated forms and full-length MAP2b and MAP2c were revealed with the anti-MAP2 antibody HM2, except for Mt, which was revealed with the antibody 46.1. Each protein was distributed uniformly in the cell body and along the processes. MAP2c and Mt induced the highest percentage of cells with multiple processes whereas MAP2b, MAP2b-1, MAP2b-2 and MAP2b-3 induced the formation of a unique process. Prob and Proc did not induce process outgrowth. Bar, 20 µm.





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