Fig. 6. Kinetochore detachment operations generated both tethered and untethered fragments. (A-E) Selected frames from a time-lapse recording of a kinetochore detachment operation. (A) Before the operation. (B) The K-fragment (white arrowhead) was detached and continued moving polewards; four acentric fragments were generated and the telomeres of two of them are located with black arrowheads. (C) The K-fragment (white arrowhead) approaches the spindle pole; the tethered, type 2 fragment (arrow points to its telomere) exhibited rapid backward motion; among the other three fragments were a weakly tethered fragment (left-pointing arrowhead) and two untethered fragments (the telomere of one is located with a right-pointing arrowhead). (D) The positions of all four fragments generated by laser microsurgery are evident: the arrow locates the telomere of the type 2 fragment, one left-pointing arrowhead locates the telomere of the weakly tethered fragment near the equator and the other two arrowheads locate the telomeres of the untethered fragments, which are in the process of being transported to the lower pole. Times are given in minutes and seconds. Bar, 5 µm (D).

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