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Fig. 5. Levels of DNA ligases III and IV are unaffected by DNA ligase I deficiency. Western analysis of protein (100 µg) extracted from mid-gestation embryos and immortalised embryonic fibroblasts. (A) Panel probed with TL25 rabbit polyclonal antibody raised against recombinant full-length human DNA ligase III. The position of the 103 kDa DNA ligase III is indicated by the arrow. (B) Panel probed with TL18 rabbit polyclonal antibody raised against a C- terminal peptide of human DNA ligase IV. The position of the 96 kDa DNA ligase IV is indicated by the arrow. The mobility of Mr markers (kDa) on the same gels is shown.





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