Fig. 5. Colocalization of DsRed-EB1 and DsRed-EB1CT but not of DsRed-EB1NT in cortical APC clusters. (a-i) Basal sections of cell extensions of cells expressing full-length DsRed-EB1 (a-c), DsRed-EB1NT (d-f) or DsRed-EB1CT (g-i). Cells were co-stained with APC antiserum (green). DsRed-EB1 predominantly localizes to the distal (plus) ends of MTs (arrows in b, c). (Fig. 4a-c) and colocalizes with APC in cortical clusters at the tip of the extension (white arrowheads in a-c). DsRed-EB1NT shows filamentous staining at MT distal (plus) ends and along MTs (arrows in e, f) (Fig. 4g-i) but does not colocalize with APC in cortical clusters (black arrowheads in d-f). DsRed-EB1CT colocalizes with APC at the tip of the extension (white arrowheads in g-i). Bar, 10 µm.

Return to article