Fig. 4. Colocation of RPA and BLM protein in pachytene spermatocytes. (A,B) The same nucleus immunostained for BLM (A) and for RPA (B). (C) The BLM image of (A) is made into a negative and superimposed on the positive RPA image of (B). Nearly all BLM foci colocate with RPA foci. The relative contributions of the two proteins to the individual foci are semi-quantifiable by the size and the intensity of the immunofluorescent signal, and this is somewhat more efficient than multiple colour combinations. (D-G) Electron micrographs of the mix of RPA 5 nm immunogold grains and 10 nm BLM immunogold grains at phosphotungstic acid-stained SCs and nodules. (H) With our antibodies, there are fewer 10 nm BLM gold grains than there are 5 nm RPA gold grains at the nodules.

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