Fig. 5. The association of MSH4p with RPA and RAD51/DMC1p. (A) The images of the red MSH4 foci and the green RPA foci are slightly offset to better demonstrate the colocation of the two types of foci. The short white lines indicate the direction of the offset and examples of colocating foci. The large bright green foci are the centromeres. (B) The colocation is also evident with immune electron microscopy. The 10 nm gold particles mark RPA antigen and the 5 nm grains mark the MSH4 antigen. The grains are associated with a transitional nodule at the central region of the SC. (C) The association of RAD51/DMC1 with MSH4 foci is less pronounced, and evidence from counts of five complete nuclei with some 150 foci each suggest that at most 10% of the foci possibly overlap and that, in general, the MSH4 foci appear after the Rad51/DMC1 foci have declined in numbers. The X chromosome at the base of (C) has, as usual, prolonged presence of RAD51/DMC1 foci. (D,E) At the EM level MSH4 foci (5 nm gold) are mostly separate from RAD51/DMC1 foci (10 nm gold). (E) The MSH4 antigen is associated with the transitional nodule, which has no evidence of RAD51/DMC1 antigen in this double-labeled preparation.

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