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Fig. 7. Time course of ANF expression in wild-type and transgenic hearts. Northern blot analysis of ANF mRNA in {alpha}MHC/Ncad and {alpha}MHC/Ecad hearts from 3-day- to 4-week-old animals (A). GAPDH signal shows loading of samples between lanes. (B) Graphic comparison of changes in ANF mRNA levels between wild-type and transgenic littermates. Note the increase in ANF expression in {alpha}Ecad heart at one week of age compared with {alpha}Ncad. ANF mRNA levels were normalized to GAPDH mRNA levels. tg, transgene; d, days; w, weeks.





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