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Fig. 1. Telomerase activity is induced by Kitl in mitotic spermatogonia. (A)
Spermatogonial cell extracts were obtained from cells that were freshly
collected (T0) or after 24 hour in culture without (control; 24 hr) or with
the addition of Kitl. Enzyme activity was assessed by the TRAP assay in the
presence (+) or absence (-) of RNase A. (B) Spermatogonial cell extracts were
cultured for 24 hours (24h) in the absence of Kitl and then for further 24
hours in culture without (48h- Kitl) or with the addition of Kitl (48h+ Kitl).
Enzyme activity was assessed by the TRAP assay in the presence (+) or absence
(-) of RNase A.