Fig. 1. Cyr1sxa2 strain in the presence of pheromone. 3 µg/ml of P factor was added at time 0 and samples were taken every hour. (A) Cells were stained with calcofluor and the sepation index was monitored. (B) The cells were imaged with a phase microscope mounted with a Hamamatsu camera and cell length was measured using NIH Image software. (C) Cells were fixed in ethanol and processed for FACS analysis. (D) The cell wall was stained with 1:1000 (from a 5 mg/ml stock in water) FITC-lectin (red), for 10 minutes; the lectin was then washed out and the cells were allowed to grow for 8 hours in the presence or absence of pheromone. Cells were then stained with calcoflour (green), which stains growing ends. The areas in red have not grown since the lectin pulse, whereas the areas in green have.

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