Fig. 7. Ability of the cells to shmoo in the presence of drugs. (A) Cells were treated with 3 µg/ml of P factor for 4 hours at 36°C. Cells were then shifted to 25°C in the presence of 200 µg/ml of LatA or DMSO, as a control, and visualised under the light microscope. 3 µg/ml of P factor was added to cells at 25°C and at 36°C. (B) Samples were taken every 2 hours, fixed in ethanol and processed for FACS. (C) After 8 hours, cells were fixed in formaldehyde and stained for actin. (D) Cells were arrested at 36°C in the presence of P factor for 4 hours; 25 µg/ml of MBC or DMSO, as a control, were added and the cells were shifted to 25°C to induce shmooing. Samples were taken every 30 minutes, fixed in formaldehyde, stained with rhodamine phalloidin to visualise actin and scored for actin localisation. Times shown are calculated from the shift to 25°C. (E) Cells treated with MBC were fixed in methanol at the end of the time course and processed for tubulin immunofluorescence to verify that no microtubules were present. Bar, 5 µm.

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