spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 8. Pom1 behaviour in the presence of pheromone. 3 µg/ml of P factor was added at time 0 to a cyr1{Delta}sxa2{Delta}pom1HA strain (A) or a cyr1{Delta}sxa2{Delta}pom1GFP strain (B). (A) Western blot of total cell extracts probed with anti-HA antibody to visualise Pom1p. (B) Pom1 GFP in live cells at time zero and after 6.5 hours in pheromone. The arrow indicates the longest shmoo, which shows Pom1GFP diffused throughout the cell. 3 µg/ml of P factor was added at time 0 to a pom1{Delta}cyr1{Delta}sxa2{Delta} cell. (C) Samples were taken every 2 hours, fixed in ethanol and processed for FACS analysis. (D) Samples were taken every 30 minutes, fixed in formaldehyde, stained with rhodamine phalloidin to visualise actin and scored for actin localisation. (E) The first 2 hours of the time course for two independent experiments (I and II) are plotted together with a wildtype control to show the rise in delocalised actin and the reduction in monopolar actin for pom1{Delta}.





Right arrow Return to article