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Fig. 1. Mammalian mitofusins are ubiquitously expressed. (A,B) RT-PCR of mitofusins (Mfn1, Mfn2) and of mitochondrial transcription factor A (Tfam) from human (A) and mouse (B) RNA. Shown are equal aliquots of the amplification reactions after 25, 30 and 35 cycles. Both mitofusins are ubiquitously expressed at similar levels. Tissues: B, brain; F, fibroblasts; H, heart; K, kidney; L, liver; M, muscle; T, Testis. (C) Dendogram of the Fzo-family. Mammalian homologs are called mitofusins (Mfn). Species: Ce, C. elegans; Dm, D. melanogaster; Hs, H. sapiens; Mm, M. musculus; Rn, R. norvegicus; Sc, S. cerevisiae; Sp, S. pombe. Accession numbers (p, protein; n, nucleotide): Ce-Fzo, p-AAC71095; Dm-FzoS, n-U95821; Dm-FzoU, p-AAF46162; Hs-Mfn1, n-AF329637; Hs-Mfn2, n-D86987; Mm-Mfn1, n-AK018181; Mm-Mfn2, n-AF384100; Rn-Mfn2, n-U41803; Sc-Fzo, n-Z36048; Sp-Fzo, p-CAA19004.





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