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Fig. 6. N-glycosylation causes an apical shift of a GFP TA construct. Filter-grown MDCK cells stably expressing GFP-22-Nglyc (A), GFP-22-MutNglyc (B) or GFP-22-MutNglyc-{Delta}myc (C) were analysed by confocal microscopy. x-z sections acquired with the fluorescein filter are shown. GFP-22-MutNglyc and GFP-22-MutNglyc-{Delta}myc are mainly basolateral, whereas GFP-22-Nglyc shows an apical localisation. Bar, 10 µm.





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