Fig. 5. Localisation of Sla1-GFP in real time. KAY401 cells were taken from a freshly struck YPAD plate and visualised as described in the Materials and Methods. The left panel shows the entire cell (at t=0) from which the subsequent images have been recorded. The images are 2D projections of 3D acquired data (16x0.4 µM optical sections) with 0.02 second exposure, taken every 3 seconds. Arrowheads denote examples of Sla1p-containing spots that remain at the same intensity or increase intensity over this time course. The arrows mark spots that decrease in intensity and disappear completely over the time course. Bar, 2 µm.

Return to article