Fig. 5. Double-epifluorescence microscopy of living MDc-2 cells stably expressing Dsc2a chimera Dsc2a. YFP, including a transiently transfected cell synthesizing, in addition, fluorescent CK 18 chimera HK 18-CFP. The picture shown is taken from a time-lapse recording (Movie 5; jcs.biologists.org/supplemental ). Fluorescence is depicted in false colors in the movie for better visualization, denoting Dsc2a. YFP-containing desmosomes in red and HK 18-CFP-positive CKFs in green. All micrographs of the movie and the picture shown in the figure consist of five superimposed focal recordings that were taken at each time point after excitation at 498 nm (YFP) and 436 nm (CFP). Bar, 5 µm.

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