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Fig. 8. Double epifluorescence microscopy of live MDc-2 cells showing the
distribution of Dsc2a.YFP and HK18-CFP during mitosis. Images at each time
point represent the projection of six pictures (z-distance of 0.5 µm)
recording the fluorescence emitted after excitation at 498 nm (YFP chimera)
and 436 nm (CFP chimera). The complete series of images is provided as Movie 8
(jcs.biologists.org/supplemental
) (recording intervals 5 minutes; same color code as in Movie 5). Note the
continued presence of Dsc2a. YFP-positive desmosomes throughout cell division,
and the considerable alterations in the HK18-CFP-containing CKF system with
some residual desmosome-associated material. Bar 5 µm.