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Fig. 11. S13A cells respond abnormally to the deduced peak and back of natural waves
generated by JH10 cells in mixed cultures. Tracks are presented of a
representative labeled S13A-1 cell and a representative unlabeled neighboring
JH10 cell in an aggregation territory that includes S13A-1 and JH10 cells in a
ratio of 1:9. JH10 cells exhibited tracks similar to those in homogeneous JH10
cell populations, suggesting that the waves relayed in the mixed population
conformed to that of the majority JH10 cell type. Note that the labeled S13A-1
cell continued to translocate in a persistent fashion, albeit at reduced
velocity, at the peak and in the back of deduced waves. Note also how the S13A
cell veers off track. The decrease in tracking efficiency was observed in a
majority of labeled S13A-1 cells in JH10 aggregation territories. Reverse
labeling experiments were performed that demonstrated that labeling did not
contribute to the observed effects.