Fig. 2. Motility is developmentally regulated in the myosin regulatory light chain phosphorylation mutant S13A. Cells were removed from developing JH10, S13A-1 and WT-res cultures at noted times, dispersed on the wall of a perfusion chamber and analyzed for cell velocity over a 10 minute period while perfused with buffer. The mean instantaneous velocity (Inst. Vel.) was computed at each time point from the average instantaneous velocity of 20 to 30 amoebae selected at random without a velocity threshold. Results similar to those for S13A-1 cells were obtained for cells of the independent mutant strains S13A-2 and S13A-3.

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