Fig. 7. Cell morphology and myosin II localization during the different phases of a simulated temporal wave of cAMP generated in the absence of a spatial gradient. A to C, D to F, and G to I represent differential interference contrast (DIC) microscopy images of representative JH10 cells fixed in the front, peak and back, respectively, of a simulated temporal wave of cAMP. A' to C', D' to F' and G' to I' are DIC images of representative S13A-1 cells fixed in the front, peak and back, respectively, of a simulated temporal wave of cAMP. J and K are representative JH10 cells in the front and the back, respectively, of a simulated temporal wave of cAMP stained with anti-myosin II antibody (first panel in each set) and scanned along the white line shown in the first panel for staining (pixel) intensity (second panel in each set). J' and K' are representative S13A-1 cells in the front and the back, respectively, of a simulated temporal wave of cAMP stained and analyzed in a fashion similar to the JH10 cells in panels J and K. Over 100 JH10 and 100 S13A-1 cells were analyzed for morphology in the front, peak and back of simulated waves, and found to exhibit the morphologies of the representative cells in this figure. Nine additional JH10 and S13A-1 cells in the front and back of simulated temporal waves were found to exhibit the distribution of myosin demonstrated for the representative cells in the figure.

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