Fig. 4. Rad53-13Myc shifts while G1 is extended. RAD53-13Myc cells were arrested with f and released following irradiation. Protein samples were collected for -Myc westerns, and p13^suc1 associated histone H1 kinase activity (A,B) was measured at the indicated times. At the same time points, additional aliquots were fixed for flow cytometry (C) or incubated for 90 minutes in trapping media (with and without f) before fixation (D). The slight delay in rad9, rad17 progression after irradiation compared with unirradiated wild type was observed only in the RAD53-13Myc background.

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