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Fig. 3. Lipid kinase activity in actin filaments and lamina-pore complexes.
Proteins from actin filaments and lamina-pore complexes (both 50 µg) from
NIH 3T3 cells were used in a lipid kinase assay as described in the legend to
Fig. 1, and the
PtdIns(4)P spots were quantified (actin filaments: s.e.m.,
n=8; lamina-pore complexes: s.e.m., n=7)
(*P<0.01). Adenosine, 200 µM; wortmannin, 1 µM;
LY294002, 10 µM.
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