Fig. 4. In vivo interaction of anti-CD scFvs with endogenous HP1ß protein. (A) COS cells were transiently transfected with DNA encoding scFv fragments and analysed after 48 hours. A western blot analysis was performed with anti-HP1ß MAC 353 of the insoluble pool from COS fibroblasts transfected with anti-ßgal scFv (lane 1). Western blot of soluble proteins from COS cells transfected with anti-ßgal (lane 2), 372nuc (lane 3) and 373nuc (lane 4) immunopurified using anti-myc 9E10 and then probed with anti-HP1ß monoclonal antibody MAC 353 is shown. The arrow points to the HP1ß protein. (B) Western blot analysis of anti-ßgal (lane 1), 372nuc (lane 2) and 373nuc (lane 3) scFv fragments immunopurified from transfected COS cells, viewed with anti-myc IgG. The migration of molecular weight markers (in kDa) is shown on the left and the heavy () and light (k) chains are indicted on the right. The arrow points to the anti-ßgal scFv fragment (lane 1) and the arrowhead points to the anti-CD scFv fragments (lanes 2 and 3).

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