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Fig. 5. Colocalization analysis of anti-CD scFvs with endogenous HP1ß, HP1{alpha}, HP1{gamma} and mPc1 (M33) proteins. Panels (A,E,I,M,Q,U) depict nuclei stained with Hoechst 33258, which preferentially stains the heterochromatin blocks present in mouse nuclei. Panels (B,F,J,N,R,V) are the same nuclei and depict the steady-state distributions of HP1 isotypes and mPc1 (M33) proteins. Panels (B) and (F) represent the distribution of HP1ß; panels (J) and (N) represent the distribution on HP1{alpha}; panel (R) represents the distribution of HP1{gamma} and (V) the distribution of mPc1 (M33). Panels (C,G,K,O,S,W) are the same nuclei and depict the distribution of the 372nuc scFv. Panels (D,H,L,P,T,X) represent the merged images. Scale bars, 5 µm.





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