Fig. 5. NuMA tail IIA localises to spindle poles in mitotic cells and induces microtubule asters, as well as multipolar and asymmetric spindles. (A) Cells expressing GFP-NuMA tail fragments (tail I, tail II, tail IIA or tail IIB, green) fixed in mitosis and processed for immunofluorescence of tubulin (red). Chromosomes are stained with DAPI (blue). The frequencies of individual phenotypes are indicated on the right (also see text). (B) Mitotic cell expressing GFP-NuMA tail IIA (green), immunofluorescence staining of endogenous NuMA (red). (C) Bacterially expressed hexa-histidine-tagged fusion proteins of NuMA tail II (left) or tail IIA (right) induce microtubule asters when incubated in Xenopus egg extracts. Bars, 10 µm (A,B); 20 µm (C).

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