Fig. 8. Loss of centrosome MTOC function in PtK2 cells injected with anti-CEP110 antibodies at mitosis. Control, cells injected with normal serum (a,b); CEP110, cells injected with anti-CEP110 antibodies (c,d). (a,c) The injected cells revealed by staining with secondary antibodies. (b,d) The injected cells were stained with anti-ß-tubulin antibodies to illustrate microtubules. Arrows denote microtubule nucleation from the centrosomes in the control cells. (Inset) Higher magnification of the centrosome from the upper cell. The arrowhead points to microtubules. Panel b has been printed with high contrast to better illustrate the microtubules growing from the centrosome. Abundant microtubules are present in the cytoplasm of control cells and increase over time following drug reversal. Bar, 10 µM.

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