Fig. 3. Keyhole formation is disrupted in kropf P mutants. (A,B) Wild-type embryos stained with anti-Wingless antibody. (C,D) Magnifications of the keyhole region shown in A,B. (A) Stage 14 embryo. Note the striped Wingless expression domain prior to keyhole formation (arrow; magnification in C). (B) Stage 15 embryo. Note that the Wingless expression domain is split (arrow; magnification in D). (E,F) Anti-Wingless antibody staining of kropf mutant embryos. (E) Stage 14 embryo. Note that the striped Wingless expression domain is not affect in kropf mutants (magnification in G) when compared with wild type. (F) Stage 15 embryo. Note that the Wingless expression domain fails to split (arrow; magnification in H). (I,J) Confocal images of anti-Fkh (green)/anti-Dve (red) staining of stage 17 wild-type (I) and kropf mutant embryos (J). Although in wild type, the ectodermal cells (ec) have invaginated into the endodermal proventriculus pouch, the ectodermal cells are stuck on top of the endoderm in kropf P mutants (compare with the larval phenotype in Fig. 2D).

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