Fig. 1. LTD₄-induced proliferation of Int 407 cells. Cells were pre-incubated in the absence or presence of GF109203X (GFX; 30 µM for 30 minutes), PD98059 (50 µM for 30 minutes), FTI-277 (20 µM for 48 hours), PTX (500 ng/ml for 2 hours) or transfected with N17 Ras. Thereafter, the cells were incubated in the absence or presence of 80 nM LTD₄ or 100 ng/ml EGF in the absence or presence of the indicated inhibitor for 48 hours. After 48 hours, the proliferative responses were determined. In (A), the conversion of tetrazolium into formazan was measured by analyzing the absorbance of formazan at 490 nm. The absorbance values are given as percentages relative to untreated cells. (B) outlines the results of the cell count, and the values are given as percentages relative to untreated cells. The data in both panels represent means±s.e.m. of four separate experiments. Statistical significant effects of the inhibitors (compared with untreated cells) were evaluated using the unpaired Student's t-test, ^*P<0.05 and ^**P<0.01.

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