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Fig. 4. DdNek2 resides at the centrosome throughout the entire cell cycle.
Fluorescence microscopy of Dictyostelium AX2 cells labeled with
anti-DdCP224 antibodies (A-A'') and GFP-DdNek2 cells (B-B''). Cells
were fixed with methanol. The secondary antibody in (A-A'') was
Cy3-labeled anti-mouse IgG. Nuclei were stained with
4,6-diamidino-2-phenylindole (DAPI). Bar, 2 µm.
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