Fig. 8. Effect of simultaneous inhibition of UPA and CK2 activities on DNA synthesis in PDGF- or bFGF-stimulated vascular SMC. Growth arrested cells were exposed to PDGF (A) or bFGF (B) in the presence of 0, 4, 6, 8 or 10 µM 4,5,6,7-tetrabromobenzotriazole (TBB) with or without the simultaneous presence of p-aminobenzamidine (p-AMB) at doses of 100 and 200 µM. Incorporation of BrdU was determined between 0 and 24 hours of stimulation with PDGF or bFGF and expressed as BrdU-labeling index. Results, given as box-and-whisker plots, are obtained from triplicate determinations in a minimum of four independent experiments. BrdU-labeling index in PDGF- or bFGF-stimulated cells was significantly reduced in all groups receiving TBB with or without p-AMB (Kruskal-Wallis test and multiple comparison's criterion for pairwise comparisons, P<0.01 for all groups receiving TBB and/or p-AMB compared with groups receiving vehicle alone). Groups treated simultaneously with TBB and p-AMB had significantly lower BrdU-LI than groups treated with TBB alone (Kruskal-Wallis test and multiple comparison's criterion for pairwise comparisons, P<0.01 for all comparisons between TBB/p-AMB-treated groups versus the corresponding TBB-treated groups unless inhibition was complete with TBB alone).

Return to article