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Fig. 4. Necturus erythrocytes loaded with fluo-4 (10 µM), bathed in a hypotonic (0.5x) Ringer solution and exposed to FITC filtered UV light. (A,C) Hexokinase (2.5 U/ml, n=6) and suramin (100 µM, n=6) inhibited the level of fluorescence normally observed following hypotonic shock. (B,D) Addition of A23187 (0.5 µm0 to the extracellular medium increased fluorescence in the presence of hexokinase (n=6) or suramin (n=6).





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