Fig. 5. Quantification of DNA abundance in the DHFR oriß region by real-time PCR. (A) Map of the DHFR initiation zone, containing the ß, ß' and initiation sites and encompassing the DHFR and 2BE2121 genes. The arrows represent the location of the amplification product using a primer set within the oriß region. (B) Quantitative realtime PCR using the LightCycler instrument, using a primer set within the oriß region or 17 kb downstream from the DHFR gene (AF028017), with DNA template extracted from the immunoprecipitation with anti-Ku70, anti-Ku86, anti-clone 162 antibodies or NGS from crosslinked or untreated CHO K1 or xrs-5 cells. Each bar represents two experiments, and one standard deviation is indicated.

Return to article