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Fig. 6. A3/4 binding activity in CHO K1 and xrs-5 cell extracts. (A) Nuclear (N) cell extracts from CHO K1 or xrs-5 cells were mixed with a radiolabeled double-stranded A3/4 DNA probe. HeLa nuclear (N) and cytoplasmic (C) extracts were used as a positive control (HeLa NC). Following the binding reaction, clone 162 or control antibody was added to the mixture, as indicated. The DNA-protein complexes were separated by 6% PAGE. The Ku70/Ku86-A3/4 (*), Ku70/Ku69-A3/4 (**) and the supershifted complexes (***) are indicated. (B) As in panel A except that cytoplasmic (C) cell extracts from CHO K1 and xrs-5 cells were used.





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