Click on image to view larger version.

Fig. 6. A3/4 binding activity in CHO K1 and xrs-5 cell extracts. (A)
Nuclear (N) cell extracts from CHO K1 or xrs-5 cells were mixed with
a radiolabeled double-stranded A3/4 DNA probe. HeLa nuclear (N) and
cytoplasmic (C) extracts were used as a positive control (HeLa NC). Following
the binding reaction, clone 162 or control antibody was added to the mixture,
as indicated. The DNA-protein complexes were separated by 6% PAGE. The
Ku70/Ku86-A3/4 (*), Ku70/Ku69-A3/4 (**) and the
supershifted complexes (***) are indicated. (B) As in panel A
except that cytoplasmic (C) cell extracts from CHO K1 and xrs-5 cells
were used.