Fig. 1. Depletion of RCC1 does not affect the nucleolar localization of U3 snoRNA in tsBN2 cells. Individual cells are observed in the differential interference contrast images (DIC), and the arrowheads point to distinct nucleoli. Also shown are the nuclei of these same cells stained with the DNA-specific dye 4,6-diamino-2-phenylindole(DAPI). U3 snoRNA was detected by fluorescence in situ hybridization (U3 snoRNA) in tsBN2 cells maintained for 6 hours at the permissive temperature (33°C) or the non-permissive temperature [40°C, resulting in RCC1 depletion (Ohtsubo et al., 1987)]. Immunofluorescence with antibodies against the trimethyl guanosine cap (TMG) of U snRNAs was also performed simultaneously and detected with Cy2-tagged anti-rabbit antibodies (TMG panel).

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