Fig. 2. Calcium binding to 34 kDa and 34 kDa EF2 proteins measured by equilibrium dialysis. (A) Calcium binding. Approximately 10 µM 34 kDa and 34 kDa EF2 proteins were dialyzed as a function of [Ca⁴⁵]CaCl₂ concentration in buffer as described in Materials and Methods. The moles of calcium bound per mole of 34 kDa protein are plotted versus the free calcium concentration for each protein. 34 kDa protein (); 34 kDa EF2 protein ([UNK]). (B) Scatchard plot from equilibrium dialysis. The moles of calcium bound per mole of 34 kDa protein divided by the free calcium concentration are plotted versus moles of calcium bound per mole of 34 kDa protein. The straight line indicates the best fit line yielding Kd of 2.4 µM.

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