Fig. 3. Binding of the 34 kDa and 34 kDa EF2 proteins to F-actin measured by co-sedimentation. (A) Moles of 34 kDa protein bound per mole of actin subunits in filaments is plotted as a function of the total 34 kDa protein added. 34 kDa protein (); 34 kDa EF2 protein ([UNK]). The 34 kDa EF2 protein binds F-actin moderately with a slightly greater affinity than the wild-type protein. (B) The relative amount of 34 kDa protein bound to F-actin in the presence and absence of micromolar concentrations of calcium. The amount of wild-type 34 kDa protein bound to F-actin decreases by 70% in the presence of micromolar calcium whereas the amount of 34 kDa EF2 protein bound to F-actin is calcium insensitive.

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