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Fig. 7. Fluorescence images of amoebae used in ratio mapping. Wild-type AX2 cells were stained with Oregon-green-labeled (A) and TRITC-labeled phalloidin (B) for the F-actin/F-actin ratio map. 34-kDa-null cells were rescued with either wild-type 34 kDa protein (C,D), 34 kDa GFP (E,F) or 34 kDa {Delta}EF2 (G,H). Cells were stained with Oregon-green-labeled phalloidin (C,E,G) and monoclonal antibody B2C against 34 kDa protein followed by a TRITC anti-mouse secondary antibody (D,H) or viewed directly for fluorescence from 34 kDa GFP (F). (C) F-Actin, (D) 34 kDa protein, (E) F-Actin, (F) 34 kDa GFP, (G) F-Actin, (H) 34 kDa {Delta}EF2. Bar, 5 µM.





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