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Fig. 4. Ectopic expression of the Op18 N-terminus is sufficient to partially
destabilize the interphase MT system in KA8 cells. (A) Cells were transfected
with the indicated NR-helix derivative or vector alone (Vec-Co) and induced
for 6 hours as in Fig. 3. The
fraction of polymerized tubulin was determined as described in Materials and
Methods and the mean of two independent transfection experiments is shown. (B)
A histogram derived from flow cytometric analysis of MT content in cells
induced to express wild-type Op18 and Op18(1-57)-NR-helix or vector alone for
6 hours as in A. Open graphs depict 
-tubulin-specific fluorescence of
cells transfected with the indicated pMEP4 derivative and the filled graph
show control staining, in the absence of anti--tubulin but in the
presence of fluorescein-conjugated rabbit anti-mouse immunoglobulin. The data
are representative for three independent experiments.
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