Fig. 11. nud-1 RNAi in C. elegans: absence of midzone microtubules and abortive cytokinesis. Fluorescent images from embryos displaying anaphase-stage microtubules in a one-cell-stage wild-type embryo (A-C) and nud-1 RNAi embryos (D-L). The embryos were stained with anti-tubulin antibodies (green) (A,D,G,J-L) and counter-stained with DAPI (blue) (B,E,H,J-L). Merged images are shown in C,F,I-L. Embryos are aligned as described in Fig. 9. (A-C) Anaphase-stage wild-type embryo with midzone microtubules. (D-I) nud-1 RNAi one-cell-stage embryos during anaphase. Midzone microtubules are absent (D,F) or weak (G,I). Weak midzone microtubules usually correspond with the presence of chromatin bridges during anaphase (H). NUD-1-depleted embryos continue the cell cycle without cytokinesis (J-L). Bar, 5 µm in all panels.

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