Fig. 2. Remodelling of mitochondria of fis1 cells upon glucose repression. fis1 mutants expressing matrix-targeted GFP were grown in glycerol to log phase and transferred for 70 minutes into glucose-containing medium. (A-C) Beam-scanning confocal images of representative budding fis1 cells as in Fig. 1A-C. (D-I) Confocal time-lapse microscopy recording the reorganization of the fis1 mitochondrial network upon glucose repression. Selected maximum intensity projections are displayed. The underlying data-stack comprises 99 three-dimensional stacks with 2200 single optical sections. Solid arrows indicate sites of future matrix separation. Feathered arrows indicate future fusion sites. At time point zero, the mitochondrial compartment resembled a large fenestrated net. Bars, 2 µm (A-C) and 1 µm (D-I). The complete dataset is available in two movies (Movie 3 and Movie 4 at jcs.biologist.org/supplemental).

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