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Fig. 3. Fission of the outer membrane in fis1{Delta} cells. (A-C) fis1{Delta} mutants expressing matrix-targeted DsRed and outer-membrane-targeted OM45-GFP were grown in PMGal to mid-log phase. After embedding in 1% agarose, live cells were imaged by beam-scanning confocal microscopy. (A) OM45-GFP, (B) DsRed in matrix and (C) bright field image. Displayed are single optical sections. Note the separation of the matrix without fission of the outer membrane (arrows). Insets: Maximum intensity projections of the separation site. (D-J) fis1{Delta} mutants expressing inner-membrane-targeted DsRed and outer-membrane-targeted OM45-GFP were grown in PMGal to mid-log phase. Displayed are maximum intensity projections. D,G,I: OM45-GFP; E,H,J: DsRed in inner membrane; F: bright field image. Time-lapse images (D-J) were taken every 2 minutes. The arrows point to a remodelling event involving complete tubule fission. Note the colocalization of the inner and outer membrane labels. Bars, 2 µm.





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