Fig. 4. Frequency of matrix separation and fusion events during remodelling upon glucose repression. (A) All detectable separation and fusion events of the GFP labelled matrix in a single wild-type cell and a single fis1 cell were counted over a time frame of 105 minutes. Only events in the mother cell were recorded. At time point zero, the mitochondrial morphologies were of a typical glycerol type. At the end of the analysis both networks were clearly simplified. (B,C) Separation and fusion frequencies at different time points following change of carbon source. (B) Wild-type cells and (C) fis1 cells. The number of matrix separations and fusions was counted at three 15 minute time frames, namely before recognizable simplification (period 1), after final separation of mother and daughter mitochondrial compartments (period 3) and in between (period 2). In the case of fis1, period 3 was defined as the point of time when no further size reduction of the fenestrated net could be observed. Only events in the mother cells were recorded. Control: (B) Wild-type cell grown to log phase in YPD and transferred to fresh YPD medium.

Return to article