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Fig. 1. Stable cell lines expressing GFP-SMN show normal nuclear morphology. Three-dimensional projections of deconvolved serial sections showing cells from line GFP-SMNE10.3 (green) counterstained with antibodies to Sm proteins (A-C), coilin (D-F) and SIP1/Gemin2 (G-I). GFP-SMN co-localises in Cajal bodies with Sm proteins (arrows in C), coilin (arrows in F) and SIP1/Gemin2 (arrows in I). Sm-containing speckles are also visible (arrowheads in C). Bar, 10 µm.





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