Fig. 6. SMN and coilin show different rates of flux through the Cajal body. (A) Single confocal sections of a cell from line YFP-coilinE1.1.1. The bleached area (yellow ring) contains a Cajal body (red arrow). The image series and the colour graph show recovery of the bleached body compared to the intensity of an unbleached body (green arrow) in the same nucleus. The greyscale graph shows averaged data from bleached CBs in three separate cells. (B) Single confocal sections of a cell from line GFP-SMNE10.3. The bleached area (yellow ring) contains a Cajal body (red arrow). The image series and the graph show recovery of the bleached body compared to the intensity of an unbleached body (green arrow) in the same nucleus and an unbleached body (blue arrow) in a neighbouring nucleus. The greyscale graph shows averaged data from bleached CBs in three separate cells. (C) Three-dimensional projection of series of confocal sections through a cell from line GFP-SMNE10.3. The bleached area (yellow box) contains a Cajal body (green arrow). The image series and the graph show recovery of the bleached body compared to the intensity of an unbleached body (green arrow) in the same nucleus and an unbleached body (blue arrow) in a neighbouring nucleus. (D) Three-dimensional projection of series of confocal sections through a cell from line GFP-SMNE10.3. The bleached area (yellow box) encompasses a large region of cytoplasm. The image series and graph show the progressive loss of signal from Cajal bodies in the bleached cell over successive bleaches (green, red and blue arrows) compared to a Cajal body in a neighbouring cell (pink arrow).

Return to article