Fig. 9. Dynamitin overexpression inhibits CCH-stimulated recruitment of DIC, p150^Glued and VAMP2 to the subapical region. Resting and CCH-stimulated (100 µM, 15 minutes) lacrimal acini transduced with Ad-LacZ or Ad-Dynt were fixed and processed for detection of p150^Glued (green, A-A',C-C',E-E',G-G') or DIC (green, B-B',D-D',F-F',H-H') with actin filaments (red, A-D,A'-D') or VAMP2 (red, E-H,E'-H') and imaged by confocal fluorescence microscopy. (A-H,A'-H') Resting and CCH-stimulated (100 µM, 15 minutes) acini, respectively. A-A', B-B', E-E' and F-F' were transduced with Ad-LacZ and C-C', D-D', G-G' and H-H' were transduced with Ad-Dynt. Arrowheads, punctate labeling; arrows, colocalization of DIC or p150^Glued with VAMP2; asterisk, lumenal regions. Bar, 10 µm.

Return to article