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Fig. 12. Dynamitin overexpression modifies CCH-independent and CCH-dependent release of bulk protein and ß-hexosaminidase. Lacrimal acini cultured on Matrigel-coated 24-well plates were transduced with Ad-GFP or Ad-Dynt-GFP, treated without or with CCH (100 µM, 30 minutes) and processed for measurement of basal (Bas), total (Tot) and stimulated (Stim) protein and ß-hexosaminidase release as described. n=6 preparations; *P<=0.05; A.U., arbitrary fluorescence unit.





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