Fig. 4. CCH increases dynein and dynactin colocalization with VAMP2 in the subapical region of acini. Lacrimal acini were fixed and processed to label DIC, p150^Glued or Arp1 with rab3D or VAMP2 and imaged by confocal fluorescence microscopy. (A-L) Resting acini, whereas A'-L' depict CCH-stimulated (100 µM, 15 minutes) acini. A-C and A'-C' show DIC (green) and VAMP2 (red) separately (A,A',B,B') and overlaid (C,C'); D-F and D'-F' show Arp1 (green) and VAMP2 (red) separately (D,D',E,E') and overlaid (F,F'); G-I and G'-I' show p150^Glued (green) and VAMP2 (red) separately (G,G',H,H') and overlaid (I,I'); and J-L and J'-L' show p150^Glued (green) and rab3D (red) separately (J,J',K,K') and overlaid (L,L'). Arrows and arrowheads, regions of colocalization of dynein or dynactin with VAMP2 or rab3D, respectively. Asterisk, lumenal regions. Bar, 10 µm.

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